P-104: Effects of Prenatal Exposure to Electromagnetic Fields on Fertility of F1 Generation in In Vitro

Background: Electromagnetic fields interact with human tissues and may have adverse effects on fertility and reproduction. During the last decades there has been a growing concern on the effects of electromagnetic radiations on biological systems in general. Radiation risks from electromagnetic fields generated by processes such as reduced fertility and a significant decrease in implantation rates and live births and incidence of congenital anomalies have been reported. This study evaluated the effects of electromagnetic fields in the prenatal period on fertility of F1 generation in in vitro. Materials and Methods: In treatment group pregnant Wistar rats were exposed 3mT EMF for 21 days, 4 hours per day, in sham group pregnant rats were kept in similar condition but off the electromagnetic field and in the control group pregnant rats were maintained in the room condition. After the pups were kept until maturity, then in vitro fertilization (IVF) in Universal IVF Medium was performance, fertilized eggs were preserved in oven CO2, 37°C. After 24 and 48 hours the number of multicellular embryos were assessed by light microscope. Results: The results showed percentage of multicellular embryos in treatment group compared with the control were decreased, but no difference observed between sham and control groups. Conclusion: Infertility is one of the major problems in match’s life. The results of present study showed that rats exposed to magnetic fields during the development period at time puberty have impaired fertility and their fertility decrease. Due to fertility decrease were observed in this study, pregnant maternal exposure in magnetic field may be cause of sperm DNA damage that it regarded as a potential risk factor to the development of normal embryos leading to impaired embryonic development.